Aggregation of human blood platelets by immune complexes in the sedimentation pattern test
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Aggregation of human blood platelets by immune complexes in the sedimentation pattern test by Gunnar MyllylaМ€

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Published by Munksgaard in Copenhagen .
Written in English

Subjects:

  • Blood -- Sedimentation.,
  • Blood cells.,
  • Blood platelet aggregation.,
  • Immunology -- Technique.

Book details:

Edition Notes

At head of title: From the Department of Virology, University of Helsinki, and the Finnish Red Cross Blood Transfusion Service, Helsinki, Finland.

StatementGunnar Myllylä.
SeriesScandinavian journal of haematology., 19
Classifications
LC ClassificationsQR187.S4 M94
The Physical Object
Pagination56 p. :
Number of Pages56
ID Numbers
Open LibraryOL5240548M
LC Control Number75311831

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Corpus ID: Aggregation of human blood platelets by immune complexes in the sedimentation pattern test. @article{MyllylAggregationOH, title={Aggregation of human blood platelets by immune complexes in the sedimentation pattern test.}, author={Gunnar Myllyl{\"a}}, journal={Scandinavian journal of haematology. Macanovic M, Lachmann PJ. Conglutinin binding polyethylene glycol precipitation assay for immune complexes. Clin Exp Immunol. Nov; 38 (2)– [PMC free article] Myllylä G. Aggregation of human blood platelets by immune complexes in the sedimentation pattern test. Scand J Haematol Suppl. ; –Cited by: Myllylä G. Aggregation of human blood platelets by immune complexes in the sedimentation pattern test. Scand J Haematol Suppl. ; – Penttinen K, Vaheri A, Myllylä G. Detection and characterization of immune complexes by the platelet aggregation test. I. Complexes formed in vitro. Clin Exp Immunol. Mar; 8 (3)–Cited by: Myllylä G. Aggregation of human blood platelets by immune complexes in the sedimentation pattern test. Scand J Haematol Suppl. ; – Hallberg T. In vitro cytotoxicity of human lymphocytes for sensitized chicken erythrocytes is inhibited by sera from rheumatoid arthritis patients. Scand J Immunol. ; 1 (4)–

Micromethods for laboratory investigations are advantageous because only small amounts of reagents are necessary, thus making larger experiments possible. This paper describes a micromethod for platelet aggregation, based on the sedimentation pattern of cells. The platelets were suspended in phosphate-buffered sodium chloride solution free of calcium or magnesium.   A rapid test for detection of circulating immune complexes in a small serum sample was developed to facilitate clinical diagnosis of immune complex disorders. The test is based on a selective precipitation of soluble circulating complexes of antigen-antibody in % concentration of high-molecular polyethylene glycol.   The plates were stored in a refrigerator (4°C) for 16 to 20 hours and subsequently the sedimentation pattern of the platelets was evaluated. A smooth white button indicated a negative result. Various ring formations (+ or ++) or an even pattern on the bottom of . Activated T lymphocytes (Ia+ T-cells) in peripheral blood are associated with immune stimulation. We found the percentages of these cells significantly increased in 95% of rheumatoid arthritis (RA) patients. Our studies showed no relation between the percentages of these cells in peripheral blood and concurrent erythrocyte sedimentation rates or rheumatoid factor titers.

Detection and characterization of immune complexes by the platelet aggregation test. II. Circulating complexes. Clin. Exp. Immunol., 8, PubMed Google Scholar. Aggregation of human blood platelets by immune complexes in the sedimentation pattern test. Scand. J. Haematol.   G. MyllylüAggregation of human blood platelets by immune complexes in the sedimentation pattern test Scand J Haematol, 19 (suppl) (), pp. l Google Scholar. The ciq binding test for the detection of soluble immune complexes. In: B.R. Bloom and J.R. David: In vitro methods in cell-mediated and tumor immunity. Academic Press, New York, p. , Immune reactions of human blood platelets. I. A comparative study on the effects on platelets of heterologous antiplatet antiserum, antigen-antibody complexes, aggregated gammaglobulin and thrombin. Thromb Diath Haemorrh. Nov 15; 20 (1)– Myllylä G. Role of platelets in immune-complex-induced aggregation of human blood platelets.